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elisa method  (Cusabio)


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    Cusabio elisa method
    CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level <t>of</t> <t>Angptl4</t> , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by <t>ELISA.</t> I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
    Elisa Method, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/elisa+method/pmc13014975-171-8-10?v=Cusabio
    Average 93 stars, based on 3 article reviews
    elisa method - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions"

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.103015

    CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.
    Figure Legend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

    Techniques Used: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay



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    Image Search Results


    CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

    Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

    Techniques: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay